In this study, the relationship between death anxiety, life satisfaction, and psychological well-being levels of individuals in middle adulthood was examined. The study group of the study consists of 340 volunteer individuals between the ages of 40-59, selected through the convenience sampling method. The data of the research were collected using the Death Anxiety Scale (DAS), Life Satisfaction Scale (LSS), and the Psychological Well-being Scale (PWS). The data collected in the study were analyzed using the SPSS-21 package program. Independent Groups T-Test, One-Way ANOVA, Pearson Product- Moment Correlation, and Multiple Linear Regression analyses were used for data analysis. According to the results obtained; While death anxiety does not show a significant difference with respect to age, it varies significantly according to gender and education level. Life satisfaction, on the other hand, does not show a significant difference with respect to age, gender, or education level. However, psychological well-being shows a significant difference with respect to age but does not differ significantly according to gender or education level. The analysis conducted using Pearson’s Correlation Coefficient revealed a weak level of relationship between death anxiety and psychological well-being, while a strong level of relationship was found between life satisfaction and psychological well-being. Multiple Linear Regression analysis indicated that death anxiety and life satisfaction significantly predict psychological well-being.